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KMID : 0903620000410020197
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Journal of the Korean Society for Horticultural Science
2000 Volume.41 No. 2 p.197 ~ p.200
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Regulation of Organogenic Ability in Gladiolus Callus by 2,4-9 Optimum Concentration Selection System In Vitro
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Abstract
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Days of adventitious root formation from gladiolus ´Topaz´ callus was longest as 23.5 days in 5th days of subculture, and the shortest as 3 days in 60th∼70th day of subculture. Then it was getting longer after that. The percentage of adventitious root formation was 100% to 6th subculture. Days for adventitious root formation was 18.9 days in first subculture and 6.4 days in 4th. Adventitious root was not formed at all after 12th. However, when the callus of in and after 7th was transplanted to the new redifferentiation medium (2,4-D free medium), the organogenic ability was recovered to 100%. These suggest that the drop of the organogenic ability in gladiolus callus is related to the accumulation of 2,4-D in the callus during subculture. To minimize accumulation of 2,4-D in the callus culture, the optimum concentration selection system was devised and applied in the gladiolus callus culture. With the system it was found that both adventitious roots and shoots were formed in the 1 to 7 fractions (0∼0.046 ㎎·L^(-1)), and only adventitious root in the 8 to 27 fractions (0.054∼0.2 ㎎·L^(-1)). But, in the 28 fraction (0.215 ㎎·L^(-1)) and above, the organ formation was not observed, i.e. only callus was proliferated. It is possible to select the optimum concentration of 2,4-D in accordance with culture purpose of gladiolus callus with this system.
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